【摘要】 目的:观察去除槟榔碱干预后血管内皮细胞(EC)增殖和分泌一氧化氮(NO)的变化。方法:采用MTT法检测去除槟榔碱干预后的EC存活率,硝酸还原酶法检测EC分泌NO量。结果:30μg/ml～90μg/ml槟榔碱干预EC24h,去除刺激并继续孵育48h,EC的增殖能力可恢复,且分泌NO增多(p<0.05);但120μg/ml槟榔碱干预24h、30μg/ml～120μg/ml槟榔碱干预48h并继续孵育48h,EC的增殖能力难以恢复(p<0.05)。结论:去除低浓度、短时间槟榔碱干预后EC增殖能力的恢复可能与具有自我保护效应的NO分泌量增加有关。更多还原

【Abstract】 Objective:To study the changes of endothelial cel(lEC)proliferation and NO production after stimulat-ed by arecoline.Method:Cell survival rate was detected by MTT assay.The NO level of the arecoline stimulated endothe-lial cell supernatan(tAECS)was measured.Result:The proliferation changes of EC induced by arecoline(30 μg/ml～ 90 μg/ml)were reversible for only 24 h following 48 h of recovery,but the survival rate of EC decreased after exposure to arecoline(120 μg/ml)for 24 h and arecoline(30 μg/ml～120 μg/ml)for 48 h(P<0.05).The NO production of AECS was increased after exposure to arecoline for only 24 h following 48 h of recovery(P<0.05).Conclusion:EC can re-cover and deplete NO after exposure to arecoline during a short-time culture,the depletion of NO may be a mechanism of EC self-protection.更多还原