【摘要】 目的:探讨一氧化氮(nitric oxide,NO)在机械力调节骨缝生长改建过程中早期表达变化的意义。方法:取实验组和对照组体外器官培养的培养液,用NO试剂盒,检测牵张实验组和对照组新生大鼠颅骨骨缝组织在1h、6h、24h、48h生成NO的表达变化。实验组和对照组标本进行常规HE染色和组织形态学观察。结果:实验组的骨缝被显著扩宽,缝细胞计数结果显著高于对照组。实验组在受力1h后,NO含量开始明显增加,到48h最为明显。生成的NO量明显高于对照组。结论:NO在机械力促进颅骨缝组织改建中具有重要的作用。更多还原

【Abstract】 Objective: To study the mechanism of NO in regulating the early growth of cranial suture under tensile stress. Method: The level of NO in the culture medium of experimental and control groups was calculated. HE stain was used to observe the sutural morphology change of the experimental and control groups. Result: The width of suture in the experimental groups is large than that of the control groups. The number of suture cells of experimental groups is obviously larger than that of control groups, especially osteoblast and mesenchymal cells. The level of NO in experimental group was higher than that of control group (P<0.05). The level of NO increased after 1h in experimental group, and it kept increasing along with the time. It increased remarkably at 48h (P < 0.01). Conclusion: NO plays an important role in mechanical force-induced suture remodeling更多还原