【摘要】 目的:比较HE染色和三联染色中不截骨的缝牵张成骨组织学变化情况。方法:不截骨直接三维牵张羊颧骨缝,固定期1、3、5、8周取材,进行三联染色和HE染色,与对侧空白对照标本比较,观察缝区组织变化、新骨质形成情况。结果:骨缝被牵开后1周,成纤维细胞、成骨细胞、毛细血管增生,大量胶原纤维形成、排列有序,3周时以胶原纤维为主,形成较成熟的骨小梁,周时编织骨形成,8周时未见网状纤维和弹力纤维,结构成熟完整。结论:三联染色5显示固定早期大量胶原纤维形成,染色显示新骨形成速度快。HE更多还原

【Abstract】 Objective:To explore the histological change in suture of zygomatic bone around for the zygomatic suture direct extension osteogenesis. Method:The zygomatic bones were drawn by 3-D external expansion appliance and sutures of the zygomatic were extended.The specimens were taken in 1,3,5 and 8 weeks of consolidation period,then examined histologically and compared with the blank contrasted tissues. The specimens were stained with Triplex and compared with the ones of heamatoxylin and eosin staining. Result:There were lots of fibroblasts,osteoblasts and capillary vessels in the expanded suture tissues in a week,and the fibers were observed to connect with sides of suture orderly. The both sides tissues of expanded suture were not in order.There were active bone formation in expanded side. The bone trabeculae were mature and oriented in the direction of distraction in the expanded sides of 3 weeks suture,which most of them were collagen fibers. A great amount of new woven bones were found in 5 weeks. In eight weeks,new bones were formed completely,and reticular and elastic fiber couldn’t be observed. Conclusion:Collagen fibers and new bones are observed to form rapidly in expanded side for direct sutural expansion osteogenesis.更多还原