【摘要】 以松仁为原料,经热水提取、乙醇沉淀、DE-52纤维素柱层析和Sephadex G-100柱层析分离得到4个多糖组分(PNP1-a、PNP1-b、PNP2-a及PNP3-a)。经GPC法测定,其相对分子质量依次为10.2×104、1.43×104、11.0×104和16.3×104。其中,PNP1-a由鼠李糖和葡萄糖组成,物质的量之比为1.00∶2.57;PNP1-b由阿拉伯糖和葡萄糖组成,物质的量之比为1.00∶4.03;PNP2-a和PNP3-a均由葡萄糖一种单糖组成。经酸水解、红外光谱(FT-IR)、紫外光谱(UV)和核磁共振光谱(NMR)对各组分进行结构分析,确定4种多糖均为β-构型的吡喃糖。更多还原

【Abstract】 Four polysaccharide fractions(PNP1-a,PNP1-b,PNP2-a,PNP3-a) were isolated from the kernel of Pinus koraiensis Sieb.et Zucc by hot water extraction,ethanol precipitation and purification followed by DEAE-cellulose and gel-permeation column chromatographies.The molecular weights of the 4 fractions were 10.2×104,1.43×104,11.0×104 and 16.3×104 respectively.The unit monosaccharides of PNP1-a were identified as rhamnose and glucose at the ratio of 1.00∶2.57;and the unit monosaccharide of PNP1-b was identified as arabinose and glucose at the ratio of 1.00∶4.03;PNP2-a and PNP3-a were both consisted of glucose.The structures of the fractions were analysed through FT-IR,ultraviolet and NMR spectroscopies,the results showed that monosaccharides of PNP1-a,PNP1-b,PNP2-a and PNP3-a were all of β-pyranose configuration.更多还原