【摘要】 采用硅胶、Sephadex LH-20柱等色谱手段,对新疆圆柏叶的黄酮类成分进行了研究,从中分离鉴定了9个黄酮类化合物:柏木双黄酮(Ⅰ)、柏木双黄酮4′,4-二甲醚(Ⅱ)、穗花杉双黄酮(Ⅲ)、罗汉松双黄酮A(Ⅳ)、儿茶素(Ⅴ)、槲皮素(Ⅵ)、异高黄芩素7-O-β-D-木糖苷(Ⅶ)、杨梅素3-O-β-D-葡萄糖苷(Ⅷ)和芦丁(Ⅸ)。其中化合物Ⅰ、Ⅱ、Ⅴ、Ⅶ、Ⅷ和Ⅸ为首次从该植物中分离得到,化合物Ⅷ和Ⅸ为首次从该属植物中分离得到。二苯代苦味肼基自由基(DPPH.)清除活性测试结果表明,化合物Ⅴ和Ⅵ的抗氧化活性较高,与阳性对照品抗坏血酸的接近;黄酮苷类化合物Ⅶ、Ⅷ和Ⅸ次之,而双黄酮类化合物Ⅰ、Ⅱ、Ⅲ和Ⅳ活性较差。更多还原

【Abstract】 Chemical constituents in the leaves of Sabina vulgaris Antoine were studied.Nine flavonoids were isolated and purified by extraction and different kinds of column chromatography,and their structures were elucidated as cupressuflavone(Ⅰ),cupressuflavone 4’,4-dimethyl ether(Ⅱ),amentoflavone(Ⅲ),podocarpusflavone A(Ⅳ),catechin(Ⅴ),quercetin(Ⅵ),isoscutellarein 7-O-β-D-xylopyranoside(Ⅶ),myricetin 3-O-β-D-glucoside(Ⅷ),rutin(Ⅸ).CompoundsⅠ,Ⅱ,Ⅴ,Ⅶ,Ⅷ and Ⅸ were obtained from this plant for the first time.Compounds Ⅷ and Ⅸ were isolated from Sabina genus for the first time.All of isolated compounds were tested for their antioxidative activities by 1,1-diphenyl-2-picrylhydrazyl free radical(DPPH·)scavenging assay.It was found that compounds Ⅴ and Ⅵ showed antioxidative activity close to that of positive control,L-ascorbic acid,and the flavonoids glycosides(Ⅶ,Ⅷ and Ⅸ)are more active than the biflavones(Ⅰ,Ⅱ,Ⅲ and Ⅳ).更多还原