【摘要】 将Zr(Ⅳ)固载在胶原上,制备了亲和吸附材料(Col-Zr),并以牛血清蛋白(BSA)为模型物,研究了该吸附材料对蛋白质的吸附特性。结果表明:每克胶原可以固载100 mg Zr(Ⅳ),被固载的Zr(Ⅳ)在pH值3.0~10.0范围内不会溶解脱落;pH值对Col-Zr吸附BSA有较大的影响,当pH值5.0、BSA的初始质量浓度为2.0 g/L时,吸附容量高达550 mg/g;吸附平衡符合Langmu ir方程,升高温度吸附量增加,表明其属于化学吸附过程;吸附动力学可用拟二级速率方程来描述,由拟二级速率方程计算所得到的平衡吸附量与实测的平衡吸附量非常吻合。更多还原

【Abstract】 An novel affinity adsorbent(Col-Zr) was prepared by immobilizing Zr(Ⅳ) onto collagen and its adsorption behaviors for proteins were studied by using bovine serum albumin as model.It was found that the Zr(Ⅳ) in the adsorbent can withstand the extraction of water in the pH value range of 3.0-10.0 when the immobilized amount was 100 mg Zr(Ⅳ)/g collagen.The adsorption capacity of BSA on Col-Zr was significantly affected by pH value of solution.The adsorption capacity arrived 550mg/g at optimal pH value(pH=5.0) when the initial concentration of BSA was 2.0 g/L.Adsorption capacity increased with the rise of temperature,implying that chemical reaction is included in the adsorption process.The adsorption isotherms can be well described by the Langmuir equation.The adsorption rate data can be well fitted by the pseudo-second-order rate model,and the adsorption capacity calculated by the model is consistent with that of actual measurement.更多还原