【摘要】 目的:探讨内耳纯化P0蛋白免疫豚鼠的听力学变化。方法:制备纯化内耳P0蛋白。分析20只经内耳纯化P0蛋白免疫后豚鼠的ABR、听神经动作电位(CAP)、畸变产物耳声发射(DPOAE)的检查结果。结果:20只动物经P0蛋白免疫后死亡4只,余16只(32耳)动物中,7耳(22%,7/32)出现ABR反应阈升高,波Ⅰ、Ⅲ潜伏期及Ⅰ-Ⅲ、Ⅰ-Ⅳ波间期明显延长(P<0.01),但Ⅲ-Ⅳ间期无明显变化;CAP(N1)幅值均有不同程度的下降,潜伏期延长(P<0.01);DPOAE各频率的幅值无明显改变,对侧白噪声抑制效应存在,有减弱的趋势,但与对照组无明显差异(P>0.05)。结论:纯化的内耳P0蛋白是内耳重要的自身抗原之一,可导致部分豚鼠的听神经和内耳出现自身免疫性疾病。更多还原

【Abstract】 Objective:To investigate the auditory characteristics of guinea pigs immunized with purified P0 protein from inner ear of guinea pigs.Method:Purified inner ear P0 protein was made by Preparative SDS-PAGE.To evaluate the results of Auditory Brainstem Response(ABR)、Compound Action Potention(CAP)、and Distortion Product Oto-acoustic Emissions(DPOAE) in guinea pigs immunized with purified guinea pigs inner ear myelin protein P0.Result:Seven ears of the guinea pigs immunized with purified inner ear P0 protein developed hearing loss.In the ABR study,peak latencies of wave Ⅰ 、Ⅲ and the interpeak latency of Ⅰ-Ⅲ、Ⅰ-Ⅳ were elevated in this group of guinea pigs compared with the control group(P<0.01).But the interpeak latency of Ⅲ-Ⅳ did not change.In the CAP study,the threshold elevated and latency prolonged(P<0.01).No significant change of DPOAE was found in the P0-sensitized guinea pigs when compared to controls(P>0.05).Even though the contra-lateral suppressive effect had the depressed tendency,but there was no significant different when compared with the controls(P>0.05).Conclusion:Purified inner ear P0 protein is an important autoimmune inner ear antigen and can develop autoimmune disease of the auditory nerve.更多还原