【摘要】 目的:探讨灵芝三萜在口腔癌变发生发展过程中的抑制作用和机制。方法:建立金黄地鼠颊囊动态癌变的动物模型,采用免疫组化技术检测caspase-3在两组动物模型中的表达变化,并进行统计学处理。结果:在整个癌变动态观察期内,两组颊囊癌变过程中不同组织学发生情况有显著差异(P<0.01),A组病变严重程度始终低于B组。随着上皮病变程度的加剧,caspase-3的表达呈现逐步增强趋势。A组与B组相比,在正常组、上皮单纯增生组、上皮异常增生组、鳞癌的不同病损中,A组阳性率低于B组。A组与B组正常组和上皮单纯增生组相比差异均无显著性。但在上皮异常增生和鳞癌组织中A组与B组差异均有显著性(P<0.05)。结论:灵芝三萜对口腔黏膜癌发展过程中具有抑制作用并可能不是通过细胞凋亡途径发挥作用,而可能发挥直接细胞毒作用。更多还原

【Abstract】 Objective: To discuss the inhibition mechanism of ganoderma triterpenoids in the development and progression of oral cancer.Methods: We established two hamster cheek pouch dynamic cancer animal models and examined the expression of caspase-3 by immunohistochemistry technology.The data was analyzed with jmtjrj 10.34.Results: In the whole dynamic carcinogenesis observation period,the occurrence of two groups’ histological difference was significant(P<0.01).The severity in group A was always lower than that in group B.Immunohistochemical study of caspase-3 showed that its expression gradually strengthened with the development of epithelial lesions.In different lesions from normal,simple hyperplasia,dysplasia to carcinoma,the positive stainings of group A were slightly lower than those of group B.There was no significant difference in the normal and simple hyperplasia between group A and B by rank sum test.However,there was a significant difference in dysplasia and carcinoma between group A and B(P<0.05).Conclusion: Ganoderma triterpenoids can inhibit carcinoma of the oral mucosa.Ganoderma triterpenes might not play a role in apoptosis,conversely,it might exhibit directly a cytotoxic effect.更多还原