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Rh阴性血型筛查及其抗体检测的临床意义

Clinical Significance of RhD-negative Screening and the Detection of its Antibody

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【作者】 董伟群何洁詹淑芬

【Author】 DONG Wei-qun1),HE Jie2),ZHAN Shu-fen3)(1) Dept.of Blood Transfusion,The 1st Affiliated Hospital of Kunming Medical University;2) The Research Center of Stem Cells,Tissue and Organ Engineering,Kunming General Hospital of Chengdu Military District of PLA;3) Dept.of Laboratory,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,China)

【机构】 昆明医学院第一附属医院输血科成都军区昆明总医院干细胞与组织器官工程研究中心昆明医学院第一附属医院检验科

【摘要】 目的对Rh阴性血型的筛查及其抗体的检测,为临床预防溶血性输血反应和新生儿溶血病的发生提供实验室依据.方法用抗D(IgM)血型定型试剂对13 208例受检者进行Rh血型鉴定.通过盐水法、聚凝胺法、间接抗人球蛋白法检测Rh阴性血中的抗体.结果13 208例受检者中,检出Rh(D)阴性75例,其中检出Rh血型免疫性抗体5例.结论常规对Rh阴性血型进行筛查及其抗体的检测,对预防溶血性输血反应,新生儿溶血病的发生及安全输血有着重要的临床意义.

【Abstract】 Objective To provide laboratory proof for the precaution of hemolytic transfusion reaction and hemolytic disease of newborn by RhD-negative screening and the detection of its antibody.Methods The Rh(D) blood types were detected by anti-IgD(IgM) blood grouping reagents,and the antibodies were detected by Saline method,Polybrene test and Indirect antiglobulin test.Results 75 samples Rh(D)-negative were detected in 13 208 samples,including 5 samples Rh(D) blood type immunity antibody.Conclusion It is very important for Rh(D)-negative screening and the detection of its antibody to prevent hemolytic transfusion reaction and hemolytic disease of newborn and blood transfusion in safety by conventional approach.

  • 【文献出处】 昆明医学院学报 ,Journal of Kunming Medical University , 编辑部邮箱 ,2008年06期
  • 【分类号】R446.6
  • 【被引频次】3
  • 【下载频次】195
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