【摘要】 目的建立豚鼠肺微血管渗漏损伤模型,从动物水平上研究从滇产毛喉鞘蕊花提取的有效部位(CF-E,异佛司可林类似物的混合物)对微血管渗漏的影响,对其抗急性肺损伤(AL)I作用机理进行初步探讨.方法静脉注射缓激肽诱导豚鼠肺微血管渗漏,然后静脉注射伊文思蓝(EB),通过测定肺组织中渗漏的EB含量,反映微血管渗漏程度.灌胃给予豚鼠CF-E,观察受试验品对微血管渗漏的影响.结果缓激肽模型组的肺组织EB含量明显增加,证明缓激肽造成了豚鼠肺微血管渗漏反应;而CF-E低、中、高剂量组(50、100、200mg/k)g肺组织中EB含量显著低于模型组(P<0.01、P<0.05),显示CF-E对缓激肽造成的豚鼠微血管渗漏具有保护作用.结论CF-E可明显降低缓激肽诱导的豚鼠肺微血管渗漏反应,提示其通过抑制肺微血管渗漏,抑制肺水肿,从而具有ALI作用.更多还原

【Abstract】 Objective In guinea pig lung microvascular leakage damage model,effect of active ingredients (CF-E)isolated from Yunnan native plant Coleus Forskohlii on lung microvascular leakage induced by bradykinin,was investigated to reveal it’s mechanism of anti-acute lung injury(ALI).Method Intravenously inject bradykinin to induce pulmonary microvascular leakage response in guinea pigs,and then intravenously inject Evans blue(EB),through testing of EB leaking level in the lung tissue,microvascular leakage level and effect of CF-E was determined after CF-E intragastric administration.Results In Bradykinin-induced microvascular leakage model group,EB content in lung tissue was significantly increased,but was significantly decreased by CF-E(50,100,200 mg/kg)compared with control(P<0.01,P<0.05).Conclusion CF-E significantly reduced bradykinin-induced lung microvascular leakage response in guinea pig,and this might berelated to it’s prevention of lung edema and causing anti-related acute lung injury action.更多还原