【摘要】 应用基因芯片技术研究小檗碱对胰岛素抵抗卵巢颗粒细胞基因表达谱的影响。方法采用胰岛素信号转导通路调节糖代谢的关键分子——PI-3K的特异性阻断剂沃漫青霉素作用于猪卵巢颗粒细胞,人工诱导胰岛素抵抗(IR)的细胞模型;同时应用小檗碱作用在细胞模型上,作用48h后提取细胞总RNA,应用猪全基因组单通道芯片对样本基因进行筛查,得出差异表达基因数据并进行分析。结果显示,中药小檗碱作用模型细胞后差异表达基因42个。差异表达基因主要涉及的功能有:物质代谢、炎症与免疫反应、信号转导等。由此可得出结论:小檗碱可通过多种途径在基因水平上对胰岛素抵抗卵巢颗粒细胞起作用。更多还原

【Abstract】 This paper uses the DNA microarray to detect the relationship between different signal pathways in cells and explore the contribution of Ber in insulin resistance ovary granular cells. The pig ovary granulosa cells are chosen as the study object in vitro, the wortmannin, the specific inhibitor of PI-3K and the key molecule in insulin receptor signal transduction pathway regulating glucolipid metabolism, is used to induce granulose cell model of insulin resistance, then insulin-resistant cells are further treated by Ber. RNA of all the cells is extracted after 48 hour. The DNA microarray is used to determine the relationship between different signal pathways in cells. There are 42 differential genes that arescreened by DNA microarray after model cells being treated by Ber., with functions such as substance metabolism, inflammation, immunifaction and signal transduction. It is concluded that Ber can affect various signal pathways to insulin resistance granular cells.更多还原