【摘要】 为了研究植物源物质对斜纹夜蛾Spodoptera litura离体培养细胞系SL-1的凋亡诱导作用,采用倒置相差显微镜观察了印楝素、喜树碱等9种物质各自对SL-1凋亡小体的浓度效应及时序性。结果表明:印楝素0.1~5.0μg/mL和喜树碱0.5~20.0μmol/L处理SL-1,24~48h后均产生大量典型的凋亡小体;茶皂素、蓖麻碱、黄樟油、丹皮酚、烟碱、苦参碱和博落回碱0.1~20.0μg/mL处理SL-1后,整个观察期72h内均无明显凋亡小体出现,凋亡诱导作用不明显。印楝素0.75μg/mL诱导SL-1细胞凋亡,从凋亡小体判断,处理后0~36h属细胞凋亡早期,36~60h属细胞凋亡中期,60h后为细胞凋亡晚期。喜树碱5.0μmol/L诱导SL-1细胞凋亡,处理后0~24h属细胞凋亡前期,24~54h属细胞凋亡中期,54h后进入细胞凋亡晚期。初步认为印楝素和喜树碱对SL-1有凋亡诱导作用,并具有一定的浓度依赖性和时序性。更多还原

【Abstract】 In order to determine the apoptotic inductivity of botanical components to Spodoptera litura cultured cell line SL-1, the concentration and time dependence of apoptotic-induce ability of nine botanical components, including azadirachtin and comptothecin, to SL-1 was investigated by inverted phase contrast microscope. The results showed that a great deal of apoptotic bodies appeared, one of the typical morphological characteristics of apoptosis, 24-48 h after treatments with azadirachtin at a concentration of 0.1-5.0 μg/mL and comptothecin at 0.5-20.0 μmol/L; the same changes, however, did not appear in SL-1 cells within the whole observation period of 72 h after treatments with tea saponin, ricinine, sassafras, paeonolum, nicotine, matrine and macleay cordata at concentrations of 0.5-20 μg/mL. Treated by azadirachtin at a concentration of 0.75 μg/mL, the early, mid and late apoptotic stages apoptosis of SL-1 cells appeared during 0-36 h, 36-60 h and 60 h after treatment, respectively; and treated by comptochetin at a concentration of 5.0 μmol/L, these appeared during 0-24 h, 24-52 h and 54 h after treament, respectively. It is tentatively concluded that azadirachtin and camptothecin both possess the concentration-time-dependence induction of apoptosis to SL-1 cells.更多还原