【摘要】 本研究利用反转录聚合酶链式反应(RT-PCR)和快速扩增cDNA末端(RACE)技术对赤拟谷盗Tribolium casta-neum体内β1-微管蛋白(β1-tubulin)基因进行了克隆,获得的基因(EU555191)全长为1573bp,包含1344bp的完整开放阅读框(ORF)。根据该基因推导出447个氨基酸序列,理论分子量约为50KD,等电点为4.68,该序列含有2个氨基酸保守区:NNWAKGHY和RKAFLHWYTGEGMDEMEFTE,并且在该基因的5’端启动子调控区发现TATA-box保守基因序列。系统发育关系研究表明:本研究扩增出的基因与其它昆虫β-tubulin基因序列有较高的同源性,达90%左右。更多还原

【Abstract】 In this paper,a full cDNA encoding β1-tubulin from Tribolium castaneum was cloned by RT-PCR and RACE techniques and characterized further.The complete cDNA(1573bp) contains a 1344 bp open reading frame encoding 447 amino acid residues(GenBank accession no.EU555191).The predicted molecular weight of this complete amino acid is about 50 KD with an isoelectric point of 4.68.Compared with other insect tubulins,the cloned gene shares two conserved amino acid sequence motifs NNWAKGHY and RKAFLHWYTGEGMDEMEFTE.The analysis of phylogenetic relationship showed that the cloned gene has high amino acid sequences homology of about 90% with other insect tubulins.更多还原