【摘要】 目的观察白藜芦醇对家兔心房肌、乳头肌的影响。方法测定白藜芦醇对家兔离体右心房窦性频率的影响,并研究白藜芦醇在不同类型钾通道阻断剂存在情况下对心脏负性变频作用的影响;测定白藜芦醇对家兔离体乳头肌收缩力的影响。结果白藜芦醇可剂量依赖性地减慢右心房窦性频率(P<0.05),1×10-3mol/L白藜芦醇可使右心房窦性频率减慢(9.4±3.5)%;与白藜芦醇组相比,ATP敏感性钾通道(KATP)阻断剂格列苯脲(glibenclam ide,G li)可部分阻断白藜芦醇的作用,其负性变频作用由原来的(9.4±3.5)%降为(7.1±1.0)%(P<0.05),但钙激活性钾通道(KCa)阻断剂四乙胺(tetraethylammonium,TEA)、电压依赖性钾通道(KV)阻断剂4-氨基吡啶(4-am inopyrid ine,4-AP)、内向整流性钾通道(KIR)阻断剂氯化钡(barium chloride,BaC l2)、乙酰胆碱(Ach)调节钾通道(KAch)阻断剂阿托品(atropine)不能阻断白藜芦醇的负性变频作用(P>0.05)。白藜芦醇对乳头肌收缩力的影响不显著(P>0.05)。结论白藜芦醇可呈剂量依赖性减慢右心房窦性频率,其作用与开放KATP通道有关,与KCa、KV、KIR和KACh通道无关。白藜芦醇对心室乳头肌无明显的负性肌力作用。更多还原

【Abstract】 Objective To investigate the effects of resveratrol on isolated atrium muscle and papillary muscle of rabbits.Method The effect of resveratrol on sinus frequency of isolated right atrium was observed,and the changes were detecued in the presence of different potassium channels blocking agents and the underlying mechanisms were studied.Results Resveratrol reduced sinus frequency in a dose-dependent manner ranging from 10-6 to 10-3 mol/L(P<0.05).The effect of resveratrol was partly blocked by ATP-sensitive K+(KATP)channel blocker glibenclamide(Gli)(P<0.05),and negative chronotropic action was decreased from(9.4±3.5)% to(7.1±1.0)%(P<0.05).The effect of resveratrol was not blocked by calcium-activated K+(KCa)channel blocker tetraethylammonium(TEA), voltage-gated K+(Kv)channel blocker 4-aminopyridine(4-AP),inward rectifier K+(KIR)channel blocker barium chloride(BaCl2) and acetylcholine regulated K+(KAch)channel blocker atropine(P>0.05).Resveratrol could not obviously inhibit contraction force of papillary muscle.Conclusion Resveratrol can induce negative chronotropic action which mechanism may relate to the opening of KATP.Resveratrol can not obviously inhibit contraction force of papillary muscle.更多还原