【摘要】 目的:探讨丹酚酸B对输尿管梗阻大鼠肾小管上皮细胞转分化影响及作用机制。方法:雄性SD大鼠,建立单侧输尿管梗阻模型(UUO)。设假手术组、模型组、治疗组(丹酚酸B30mg·kg-1·d-1)术后第9天处死各组大鼠。采用光镜观察肾间质纤维化、炎细胞浸润,免疫组化观察肾组织转化生长因子-β1(TGF-β1)、α平滑肌肌动蛋白(α-SMA)和Vi-mentin蛋白表达的变化。结果:(1)治疗组大鼠肾间质纤维化程度较模型组明显减轻;(2)UUO模型第9天时大鼠肾组织TGF-β1、α-SMA和Vimentin表达明显增强,炎细胞浸润明显增加。采用丹酚酸B治疗后,肾组织TGF-β1、α-SMA和Vi-mentin表达的异常增强能得到有效抑制,炎细胞浸润明显减少。结论:丹酚酸B具有减轻肾组织纤维化的作用,而这一作用与其能有效抑制炎细胞浸润和TGF-β1过度表达,进一步阻抑肾小管上皮细胞转分化(EMT)有关。更多还原

【Abstract】 Objective:To investigate the effect of Salvianolic acid B on epithelial-mesenchymal transition in rat with unilateral ureteral obstruction (UUO).Methods:Male SD rats were divided into 3 groups randomly:sham operation group,UUO group,treatment group(:UUO+Salvianolic acid B per kg 30 mg/d by inragastric administration).Kidney were harvested at the 9th day after UUO.The renal tissues were examined by HE,PAS and Masson staining.Tissue TGF-β1,α-SMA and Vimentin protein expression was determined by Immunohistochemistry.Results:After UUO for 9 days,expression of TGF-β1、α-SMA and Vimentin protein,interstitial fibrosis and tubulointerstitial lesion were observed in obstructed to be more significant,compared with those in the sham operation group rats.Salvianolic acid B significantly inhibited TGF-β1,α-SMA and Vimentin protein expression and decreased interstitial fibrosis and tubulointerstitial lesion in obstructed kidney of treation group.Conclusion:Salvianolic acid B had beneficial effects on interstitial fibrosis in SD rats with UUO,and this effect may be associated with inhibition of the trans-differentiation of renal tubular epithelial cells towards myofibroblast.更多还原