【摘要】 目的:探讨中药地黄对大鼠肾纤维化的作用及可能的机制。方法:将实验动物分成正常对照组、模型组、地黄组,采用一次性尾静脉注射阿霉素复制大鼠肾病模型。治疗8周后观察尿蛋白、尿素氮、血肌酐及肾脏病理改变;同时观测肾皮质中转化生长因子β受体Ⅰ(TβRⅠ)、转化生长因子β受体Ⅱ(TβRⅡ)和Smad2、7的表达。结果:地黄组各项指标与模型组相比,均有统计学差异,形态学观察也显示其组损害轻于模型组。且模型组肾组织TβRⅠ、Smad2 mRNA显著上调,地黄组肾组织TβRⅠ、Smad2 mRNA显著下调,而模型组肾组织Smad7蛋白表达显著下调。同时TβRⅡ mRNA的表达无明显变化。结论:在阿霉素肾病大鼠模型中,地黄提取物对大鼠肾脏有一定的保护作用,其信号转导机制至少部分与它能下调TβRⅠ、Smad2及上调Smad7的表达有关。更多还原

【Abstract】 Objective:To explore the effect and mechanism of Dihuang in treating adriamycin (ADR) induced renal fibrosis rats.Methods:The Nephropthy model rats established by a single intravenous injection of Adriamycin were divided into the normal group,the model control group and the Dihuang group.24 h urinary protein excretion,albumin,serum creatinine,BUN and pathological changes of kidney were examined and the expression levels of transforming growth factor β receptor Ⅰ (TβRⅠ),transforming growth factor β receptor Ⅱ (TβRⅡ), Smad 2 and Smad 7 were determined after 8 weeks treatment.Results:After treatment,all the parameters between the Dihuang group and model group were significantly different respectively;pathological changes in the Dihuang group were milder than those in the model group.The mRNA expressions of TβRⅠ and Smad 2 in kidney tissue of the model group were significantly upregulated and those of TβRⅠand Smad 2 in kidney tissue of the Dihuang group were significantly downregulated and the protein expression of Smad 7 in kidney tissue of the model group were significantly downregulated, while the mRNA expression of TβRⅡ remained unchanged.Conclusion:Dihuang significantly prevenus against renal injury in the rats with nephropathy partially by downregulating the expression of TβRⅠ and Smad 2 and upregulating the expression of Smad 7.更多还原