【摘要】 目的:检测TSA对Ⅱ型胶原诱导的T细胞增殖的抑制作用。方法:采用CIA小鼠模型,收集引流淋巴结细胞。MTT法观察不同浓度TSA对T细胞的抑制作用,Annexin-v观察细胞凋亡情况。用Caspase 3试剂盒检测Caspase 3酶活性。结果:TSA呈时间和剂量依赖性抑制T细胞增殖,经20ng/ml TSA处理后,T细胞凋亡率增高,呈现时间依赖性效应关系。20ng/ml TSA作用T细胞后,实验组与对照组相比Caspase3活性明显升高。结论:TSA可能通过上调Caspase3活性,诱导细胞凋亡,从而抑制T细胞增殖。更多还原

【Abstract】 Objective: To examine the inhibition of TSA on T cell reproduction induced by collagen type Ⅱ. Methods: The inhibition of various TSA on T cells was observed by MTT assay. By collecting draining lymph node cells of CIA mouse. Caspase 3 activity was measured by ApoAlert CPP32 colorimetric assay kit. Results: The inhibition of TSA on T cells shows a time-dependent and dose-dependent effect. The rate of T cell apoptosis increased after being treated with 20ng/mL TSA,and shows a time-dependent effect. The activity of Caspase 3 in the test group had a significant increase after being treated with 20ng/mL TSA,compared with it in the control group. Conclusion: TSA may up-regulate the activity of caspase 3 ,and induce apoptosis,thereby inhibiting T cell reproduction.更多还原