【摘要】 目的:观察淀粉样蛋白(Aβ)1-42诱导的阿尔茨海默病(AD)大鼠模型经颅内注射Aβ1-42单克隆抗体(mAb)后其行为及脑组织病理的改变。方法:利用Aβ1-42蛋白片段诱导建立AD大鼠模型后,侧脑室内注入Aβ1-42 mAb,记录Morris水迷宫试验逃生时间作为行为学指标,同时取脑组织作切片染色,观察其病理改变。结果:AD大鼠模型颅内注射Aβ1-42 mAb后,水迷宫试验逃生时间明显缩短,表明其学习记忆能力明显改善;病理切片染色观察到脑组织内Aβ沉积斑块消失。结论:颅内注射Aβ1-42 mAb对AD动物模型有明显疗效。更多还原

【Abstract】 Objective: To observe the behavior and brain tissue variation after using amyloid peptide(Aβ1-42 monoclonal antibody(mAb) cerebral injection in Alzheimer’s disease(AD) rat.Methods: AD rat can be created with the induction of Aβ1-42 peptide.After the injection of Aβ1-42 mAb in right side ventricle,escaping time of rats in Morris Labyrinth can be recorded as the action marker.Meanwhile,pathology assay should be done with its brain tissue.Results: After cerebral injection of Aβ1-42 mAb,the escaping time of AD rat in Morris Labyrinth decreased remarkably.That means its learning and memory ability obviously improved.The result of brain tissue pathology assay show that the Aβblot disappeared.Conclusion: Aβ1-42 mAb cerebral injection do have an obvious curative effect on AD rat.更多还原