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微生物法测定抗菌素残留灵敏度的影响因素概述
Factors Influencing the Sensitivity of Microbiological Determination of Antimicrobial Residues
【摘要】 微生物法检测兽药残留的原理是利用了动物组织液中残留的抗菌药物可以抑制或杀死微生物,从而在药液周围形成肉眼可见的抑菌圈。本文综述了影响微生物法测定抗菌素残留灵敏度的主要因素,包括工作菌种的选择,菌液浓度的确定,载药量的工具,培养基的成分、厚度、pH,方法制定过程中空白组织样的选择,并阐述了几种主要微生物方法的应用。
【Abstract】 The principle of microbiological detection of antimicrobial residues is that antimicrobial agent residues could inhibit or kill microbes,so that there is a zone of inhibition around the tested animal tissue fluid.The article summarized the main factors influencing the sensitivity of the microbiological methods,such as selection of bacterial strain,definition of bacterial fluid concentration,means of carrying antibacterial agent,ingredient,thickness and pH of the medium,as well as selection of control tissue samples.The applications of several microbiological detection methods were also expounded.
【Key words】 antimicrobial agent; residue; microbiological detection; sensitivity;
- 【文献出处】 江苏农业学报 ,Jiangsu Journal of Agricultural Sciences , 编辑部邮箱 ,2008年05期
- 【分类号】S859.84
- 【被引频次】4
- 【下载频次】179