【摘要】 摘要:应用高效液相色谱,对北方某水库5个采样点藻类细胞内3种微囊藻毒素(MC-LR,RR和YR)进行了为期一年的监测.研究了细胞内微囊藻毒素随时间的变化规律,并探讨了环境因子对细胞内微囊藻毒素产生的影响.结果表明,藻类细胞内3种微囊藻毒素被检出的时间不一致,LR异构体在4月份下旬即可检测到,而RR和YR异构体则要到5月份中旬才能检测到.LR和YR异构体在9月份出现高峰值,而RR异构体在10月份达到全年的最大值.4—9月份,LR异构体含量最大,RR异构体次之,YR异构体含量最小;10—12月份,RR异构体含量最大,10月和11月LR含量大于YR,但是12月份LR未检出,而YR含量比11月份还高.环境因子对细胞内微囊藻毒素的影响也不一致:溶解氧和硝酸盐氮都与3种异构体呈显著负相关;总磷、溶解性总磷和磷酸盐都与RR异构体呈显著正相关,氨氮和LR异构体之间存在显著负相关.更多还原

【Abstract】 The high performance liquid chromatography(HPLC) was used to monitor the intracellular microcystin-LR,-YR and-RR(MC-LR,-YR,-RR) within one year in five sampling cites: in a reservoir of northern China.The rule of the intracellular microcystins changing with time was explored,and the influence of environmental conditions on intracellular microcystins level was discussed.The MC-LR is detected until the month of April.The MC-YR content is below the limit of detection from February to April,and so is that of MC-RR from January to April.The peak contents of MC-YR and MC-LR occur in September,but MC-RR occur in October.From April to September,the MC contents order is ρ(MC-LR)>ρ(MC-RR)>ρ(MC-YR).The MC-RR content is highest among the three kinds of intracellular microcystins from October to December.The MC-LR content is higher than that of MC-RR in October and November.In December,the MC-LR content is below the limit of the detection,but the MC-YR is higher than that of November.The environmental factors have different influence on intracellular microcystins level.Dissolved oxygen and nitrate are significant related factors to intracellular microcystins.Total phosphorus,dissolved total phosphorus and dissolved phosphorus correlate well with MC-RR.Ammonia nitrogen displays negative significantly correlation with MC-LR.更多还原