【摘要】 目的研究5-FU缓释微球经胃十二指肠动脉灌注对兔VX2肝肿瘤的治疗作用。方法将成功接种肝VX2肿瘤的模型兔随机分成4组,每组10只,用显微外科手术临时阻断肝总动脉血流,经胃十二指肠动脉插管至肝固有动脉起始部给药行介入治疗,术毕结扎胃十二指肠动脉。A组(生理盐水对照组),注射生理盐水0.5～1ml;B组(碘佛醇对照组)注射碘佛醇0.5～1ml;C组为碘油组(疗效对比组),注射超液化碘油0.5～1.0ml;D组为5-FU缓释微球组,注射5-FU缓释微球10mg和碘佛醇1ml混合溶液。4组实验动物于治疗1周后观察肿瘤生长情况、坏死程度,并采用原位末端标记法(TUNEL)检测肿瘤细胞凋亡指数(AI)。结果治疗1周后5-FU缓释微球组肿瘤生长受到抑制,肿瘤生长率低于生理盐水对照组和碘佛醇对照组(P﹤0.05),与碘油组差异无统计学意义(P>0.05)。4组肿瘤均有不同程度坏死,5-FU缓释微球组和碘油组肿瘤坏死率明显高于另两组(P<0.05)。生理盐水对照组、碘佛醇对照组和5-FU缓释微球组肿瘤细胞凋亡指数分别为1.69±0.18、1.75±0.27和8.03±0.63,5-FU缓释微球组与各对照组相比差异有统计学意义(P<0.05)。结论5-FU缓释微球经动脉灌注可抑制肝肿瘤生长,诱导肿瘤细胞凋亡,促进肿瘤坏死,是有效的化疗栓塞剂。更多还原

【Abstract】 Objective To study the therapeutic efficacy of 5-FU sustained-release microspheres infused through gastroduodenal artery for rabbits with VX2 liver carcinoma. Methods Forty rabbits implanted with liver VX2 tumors were randomly divided into 4 groups,with 10 rabbits in each group. All rabbits were demonstrated with liver neoplasmas by MSCT. During the open laparotomy,common hepatic artery was ligated under microsurgery temporarily,then a catheter was inserted via gastroduodenal artery under microscopy and follow by administration of therapeutic agents through the catheter. Group A (Control group 1) was given 0.5 to 1.0 ml saline; Group B (contrast agent group) was administrated with 0.5 to 1.0 ml ioversol injection; Group C (lipiodol group) received 0.5 to 1.0 ml lipiodol; Group D (5-FU sustained-release microsphere group) was infused with mixture of 10 mg 5-FU sustained-release microsphere and 1ml Ioversol. Mutislice spiral CT scan was performed to measure tumor volume on the 7th day after treatment. The tumor growth rates,necrosis rates were observed and cell apoptotic index was recorded by TUNEL. All parameter thus gained were compared among the A,B and D groups. Results One week after treatment,the 5-FU sustained-release microsphere group showed the growth of tumor was significantly inhibited,tumor growth rate lower than those of group A and group B (P < 0.05),and showed no significant statistical difference to group C(P > 0.05). The tumor necrosis rates had some differences in each group,with necrosis rates of group D and C much higher than those of group A and B (P < 0.05). The tumor apoptotic indexes (AI) were 1.69 ± 0.18 (group A),1.75 ± 0.27 (group B),8.03 ± 0.63 (group D),respectively (P < 0.05); there was significant statistical difference between groups D and group A. Conclusion The 5-FU sustained-release microspheres transarterial infusion to liver can inhibit the growth of hepatic tumor,induce the tumor cell apoptosis,aggravate the tumor necrosis,and thus is an effective chemoembolization agent.更多还原