【摘要】 目的:探讨人胚胎早期发育中生长抑素在胰及胃肠道的表达及分布。方法:采用免疫组织化学SABC法对30例7~14周人胚胎胰及胃肠生长抑素阳性细胞进行定位。结果:胚胎7周时胰导管上皮及小肠均未见有生长抑素阳性细胞;9周时,胰导管上皮可见较少的生长抑素阳性细胞,14周时生长抑素阳性细胞增多并开始聚集参与形成胰岛;11周时小肠内才出现生长抑素阳性细胞,阳性细胞分布在小肠黏膜上皮及固有层内,14周时生长抑素阳性细胞明显增多。结论:胚胎早期发育中,分布于胰导管上皮的D细胞其生长抑素的表达早于小肠的D细胞;生长抑素不仅参与胰岛细胞的分化发育,还与胃肠道的内分泌功能形成密切相关。更多还原

【Abstract】 Objective: To investigate the expression and distribution of somatostatin in the pancreas and gastrointestinal tract during early human embryonic development.Methods: Sample collection was conducted in accordance with the principle of informed consent.Histochemical S-ABC method was adopted to study the expression and localization of somatostatin in embryos at 7-14 weeks after gestation.Results: No somatostatin-positive cells were found in the pancreatic epithelium and gastrointestinal tract epithelium at embryonic week 7.At 9 weeks,a few somatostatin-positive cells were observed on the pancreatic epithelium and the number of somatostatin-positive cells increased and aggregated to form pancreatic islets at 14 weeks after gestation.Eleven weeks after gestation,there were a few somatostatin-positive cells on the mucosa epithelium and lamina propria in the small intestines and increased 14 weeks after gestation.Conclusion: The expression of somatostatin in the pancreatic epithelium priors to gastrointestinal tract epithelium in the early human embryonic development.Somatostatin is not only related to pancreatic islet cells differentiation but also to forming the endocrine function of the gastrointestinal tract.更多还原