【摘要】 目的探讨松果体摘除及褪黑素对大鼠胸腺上皮细胞白细胞介素7(IL-7)表达的影响及其意义。方法1.培养大鼠胸腺上皮细胞,应用广谱角蛋白抗体进行免疫细胞化学显色鉴定;MTT法观察褪黑素(1×10-3～10-9mol/L)干预对细胞生长的影响;细胞分空白对照组、褪黑素10-8mol/L处理组和褪黑素10-6mol/L处理组,RT-PCR法观察细胞IL-7mRNA的表达;2.选用清洁级雄性SD大鼠110只,分为正常对照组、假手术对照组、松果体摘除组、松果体摘除+褪黑素7.5mg/(kg.d)腹腔注射组和松果体摘除+褪黑素15mg/(kg.d)腹腔注射组。术后4周和8周取材,运用免疫组织化学和RT-PCR方法观察胸腺上皮细胞IL-7表达的变化。结果褪黑素干预对大鼠胸腺上皮细胞的生长无显著影响,但能使IL-7mRNA的表达水平呈剂量依赖性升高;松果体摘除对大鼠胸腺上皮细胞表达IL-7无显著影响,补充褪黑素15mg/(kg.d)4周后IL-7表达显著增高,8周后均下降至正常水平。结论松果体及褪黑素可能通过影响大鼠胸腺上皮细胞IL-7的表达,从而调节胸腺细胞的分化发育。更多还原

【Abstract】 Objective To study the effect of pinealectomy and melatonin on interleukin 7(IL-7) expression of rat thymic epithelial cells(TECs) and its physiological significance.Methods Cultured rat TECs were immunostained with anti-pan-cytokeratin;MTT was applied to investigate the growth of cultured rat TECs with melatonin treatment(1×10-3～10-9 mol/L),semiquantitative RT-PCR was applied to assess IL-7 mRNA expression.Totally 110 clean-grade male SD rats were divided into five groups:normal control group,sham operation group,pinealectomized group,pinealectomy+7.5mg/(kg·d) MLT group,pinealectomy +15mg/(kg·d) MLT group,and took the thymus out in the 4th week and the 8th week,Immunohistochemistry and semiquantitative RT-PCR were applied to observe IL-7 expression in rat TECs.Results Melatonin made no difference on rat TECs growth,but IL-7 mRNA expression in TECs was increased by melatonin treatment in a dose dependent manner;Pinealectomy made no difference on the synthesizing of IL-7 by rat TEC,but 15mg/(kg·d) melatonin treatment resulted in significant increasing of IL-7 expression in certain period.Conclusion pinealgland and melatonin may regulate the differentiation and development of thymocyte by IL-7 expression of TEC.更多还原