【摘要】 目的比较人正常前列腺(NP)和前列腺增生(BPH)中间质细胞标记蛋白、增殖细胞核抗原(PCNA)和雌激素受体α(ERα)的表达差异,并检测BPH组织间质细胞标记蛋白与PCNA或ERα同时呈阳性染色的细胞。方法对4例NP和8例BPH连续切片应用免疫组织化学方法,观察波形蛋白(vimentin)、α-平滑肌肌动蛋白(α-SMA)、肌球蛋白(myosin)、PCNA和ERα的表达定位。结果与NP相比在BPH中,α-SMA阳性染色细胞显著增加;波形蛋白在间质中阳性染色细胞有所增加,在腺泡基底层及临近腺泡外层间质中阳性染色细胞明显增加;在临近腺泡外的数层间质细胞中肌球蛋白和ERα由部分阳性变为完全阴性染色,而在远离腺泡的间质中其阳性染色细胞由散在斑块状分布变为簇状密集排列;PCNA在间质中阳性染色细胞有所增加,在基底细胞层中阳性染色细胞显著增加。连续切片免疫组织化学染色显示,在腺泡上皮基底层存在PCNA与波形蛋白、ERα共定位的细胞;在间质中存在肌球蛋白与ERα共定位的细胞。结论与NP相比,BPH间质细胞表型发生明显变化,且其增殖和表型转化与ERα表达定位的改变密切相关。更多还原

【Abstract】 Objective To investigate the different expression patterns of the phenotypic marker protein for stromal cell,proliferating cell nuclear antigen(PCNA) and estrogen receptor α(ERα) in normal human prostate(NP) and benign prostatic hyperplasia(BPH) tissue,and to locate cells stained positively with the marker protein for stromal cell and PCNA or ERα at the same position.Methods Serial sections of 4 NP and 8 BPH specimens were subjected to immunohistochemistry(IHC) staining for vimentin,α-smooth muscle actin(α-SMA),myosin,PCNA and ERα.Results Compared with that in the NP tissues,the number of α-SMA positive cells increased significantly in BPH tissue,and the number of vimentin positive cells increased moderately in the stroma and prominently surrounding the acinus and in the basal layer.In BPH tissue,the myosin and ERα staining signal was lost in the stromal cells surrounding the acinus,and the positive staining cells gathered into bunches in the stroma far away from the acinus,while the positive cells were sporadically distributed in the NP tissue.The PCNA positive cells increased moderately in the stroma and increased significantly in the basal layer.The serial sections staining results showed that there was a colocation of PCNA,vimentin and ERα in basal cells,and a colocation of myosin and ERα in stromal cells.Conclusion There are apparent phenotypic modulations of the stromal cells in the BPH specimen compared with in the normal prostate,and the proliferation and phenotypic modulation are correlated with the differential expressions of ERα.更多还原