【摘要】 目的观察马桑内酯致痫及妥泰干扰大鼠中央前回血脑屏障的超微结构改变,探讨癫痫发作及妥泰治疗机制中血脑屏障的意义。方法成年健康雄性SD大鼠30只,随机分为对照组、癫痫组、治疗组;癫痫组、治疗组用马桑内酯注入到大鼠侧脑室,制作癫痫动物模型;实验组用妥泰在癫痫发作后1h灌胃,持续7d,1次/d;7d后取大鼠大脑中央前回,做超薄切片,电镜观察中央前回血脑屏障的超微结构改变。结果癫痫组内皮细胞、基膜、周细胞出现明显水肿,电子密度降低;治疗组水肿缓解。血脑屏障内皮细胞胞质厚度、基膜厚度、周细胞胞质厚度的比较表明,癫痫组与对照组也有显著性差异(P<0.05);治疗组与癫痫组比较,有显著性差异(P<0.05)。结论1.马桑内酯致痫大鼠血脑屏障内皮、基膜、周细胞出现明显水肿,妥泰使水肿缓解;2.血脑屏障超微结构改变可能是癫痫发作及恢复的重要机制。更多还原

【Abstract】 Objective To observe the change of the precentral gyrus blood-brain barrier ultrastructure in the epilepsy rats induced by coriaria lacton and affected by topimmate,and to study the effect of the blood-brain barrier on the mechanism of the falling sickness and therapy by topimmate.Method Thirty healthy male Sprague Dawley rats were randomly divided to three groups: the control group,the epilepsy group and the treatment group.The coriaria lacton was injected into the lateral ventricles of the rats in the epilepsy and treatment groups,and the epilepsy animal model was established.Gastric perfusion was performed 1 hour after falling epilepsy to the rats of the treatment group and was repeated one time per day for 7 days.The precentral gyrus was cut 7days later,and the ultrathin sections were made.The change of blood-brain barrier ultrastructure was observed under electron microscope.Results The endothelium,basement membrane,pericyte of epilepsy group displayed obvious edema.The electron density reduced,and the edema of the treatment group alleviated.There was signifcant difference between the epilepsy group and the control group(P<0.05),in the thinkness of endothelium cytoplast,basement membrane and pericytes cytoplast of blood-brain barrier.So was the case between the treatment group and the epilepsy group(P<0.05).Conclusion 1.Endothelium,basement membrane and pericytes of blood-brain barrier of the epilepsy rats induced by coriaria lacton displayed obvious edema,and topimmate weakened the edema.2.The change of blood-brain barrier ultrastructure maybe be the important mechanism in the falling and healing of epilepsy.更多还原