【摘要】 目的探讨HOXa-10基因在小鼠胚胎着床过程中的作用。方法应用实时荧光定量PCR(FQ-PCR)分别检测未孕及早孕小鼠第1、2、3、4、57、d HOXa-10基因表达量的变化规律;同时用可以持续表达HOXa-10的DNA/脂质体复合物质粒和HOXa-10反义寡脱氧核糖核酸分别转染到受孕2d小鼠子宫角内,观察胚泡着床数。结果FQ-PCR结果显示,随着小鼠妊娠天数的增加,HOXa-10基因表达量也逐渐增高,在孕4d达到峰值,孕5d开始下降,孕7d时表达量已接近未孕状态;在用HOXa-10 DNA质粒/脂质体转染孕2d小鼠子宫角后,小鼠的胚泡着床数明显增加;用HOXa-10反义寡脱氧核糖核酸/脂质体转染孕2d小鼠子宫角后,小鼠的胚泡着床数明显减少(P<0.05)。结论在胚泡植入窗口期,子宫内膜HOXa-10基因表达上调,其可能参与了胚泡着床过程。更多还原

【Abstract】 Objective To investigate the function of HOXa-10 gene in the course of mouse blastocyst implantation. Methods Real-time fluorescence quantitative PCR was applied to detect the expression of HOXa-10 gene in normal mice and day1,day2,day3 day4,day5 and day7 pregnant mice.Plasmid containing the HOXa-10 cDNA fragment with liposome and plasmid containing HOXa-10 antisense oligonucleotides with liposome were transfected respectively into the uterine horns of day 2 pregnant mice,and the number of blastocyst-implanted was counted and compared with that of normal day 2 pregnant mouse. Results HOXa-10 gene expression in the pregnant group is higher than that of the non-pregnant group,and the results showed a gradual increasing trend as days went by: HOXa-10 gene expression was the highest on pregnancy d4,began descending on pregnancy d5 and was close to that of non-pregnancy on pregnancy d7.The number of blastocyst increased in the uterine horns which were injected with plasmid containing the HOXa-10 cDNA fragment with liposome.On the contrary,the number of blastocyst decreased in the uterine horns which were injected with plasmid containing HOXa-10 antisense oligonucleotides with liposome(P<0.05).Conclusion HOXa-10 gene is persistently expressed in mice endometrium during early pregnancy and may participate in the regulation process of mouse blastodyst implantation.更多还原