【摘要】 目的探讨体外培养诱导慢性乙型肝炎患者外周血树突状细胞(DCs)功能状态改善的方法。方法用GM-CSF+IL-4+INF-α诱生慢性乙型肝炎患者及健康人外周血来源的DCs,在乙肝患者DCs成熟前加入营养浓度的硒(0.3mmol/L)共培养。流式细胞仪(FCM)检测细胞表面CD80、CD86表达水平,四甲基偶氮唑盐比色法(MTT法)检测DCs刺激同种异体淋巴细胞增殖能力及酶联免疫吸附测定法(ELISA法)检测DCs培养上清中白细胞介素12(IL-12)的分泌水平。结果经营养浓度硒(0.3mmol/L)作用后的乙肝患者的DCs刺激淋巴细胞增殖能力明显高于未经硒作用的乙肝组(P<0.01),IL-12的分泌水平和表面刺激分子也明显高于乙肝组(P<0.01),加硒组和健康组之间则没有显著性差异。结论体外经营养浓度的硒作用后的乙肝患者的DCs可有效刺激淋巴细胞增殖反应,提高IL-12分泌水平,在一定程度上能恢复DCs的免疫功能,这些为今后DCs的慢性乙型肝炎的免疫治疗提供新思路。更多还原

【Abstract】 Objective To explore the methods of improving the immune function of dendritic cells(DCs) from patients with chronic type B hepatitis in vitro. Methods Monocytes from type B hepatitis patients and healthy controls were incubated with GMCSF and IL-4 and IFN-α to induce dendritic cells(DCs) generation.Then the nutrien-concentration sodium selenite was added for co-culture before the maturity of DCs.The samples were separated into three groups: hepatitis-B patients (group A),hepatitis-B patients with Se(group B),healthy control(group C).The phenotypes of DCs including CD80,CD86 were characterized by flow cytometry(FCM) and the response of allogeneic lymphocytes was detected by MTT assay.The concentration of cytokines in the supernatant such as IL-12 was tested by ELISA assay. Results After the nutrien-concentration Se was added for co-culture,the proliferation ability of isotype xenoma lymphocyte stimulated by DCs was obviously streng thened compared with group A,and the excretion level of IL-12 also increased(P<0.01).There was no significant difference between group B and group C.Conclusion Co-cultured with the nutrient-concentration Se in vitro,DCs could stimulate lymphocyte proliferation reaction effectively,improve the excretion of IL-12,and recover DC’s immunological function partly,which may provide a theory for future immune therapy by DCs for chronic type B hepatitis patients.更多还原