【摘要】 目的探讨胚胎干细胞-D3株(ES-D3)联合碱性成纤维细胞生长因子(bFGF)移植治疗大鼠急性心肌梗死,是否有利于心脏结构的恢复和心功能的改善。方法Wistar大鼠40只随机均分成5组,分别为正常对照组(组1)、梗死未治疗组(组2)、培养基注射组(组3)、ES-D3移植组(组4)、ES-D3+bFGF移植组(组5)。大鼠急性心肌梗死造模后1周移植体外分化并经标记的ES-D3,4周后进行心功能及组织学检测。结果ES-D3体外能分化为心肌样细胞。梗死后4周检测表明,移植细胞在大鼠体内稳定存活。心功能及组织学检测表明,组2与组3大鼠无显著差异(P>0.05)。与组2比较,组4和组5大鼠心肌梗死面积均显著减小,左心室重量减轻(P<0.01);毛细血管密度显著增高(P<0.01);左心室功能显著改善。结论急性心肌梗死后移植ES-D3可以促进大鼠心血管新生、阻止心室重构、减少瘢痕面积、显著改善心功能,联合应用bFGF可进一步获益。更多还原

【Abstract】 Objective To investigate the feasibility of recovering the cardiac structure and improving the cardiac function after the transplantation of embryonic stem cells-D3(ES-D3) combined with basic fibroblast growth factor(b-FGF) into the acute myocardial infarction(AMI). Methods Forty Wistar rats were used and randomly divided into 5 groups equally,including the normal control group(group 1),the myocardial infarction without treatment group(group 2),the medium injected group(group 3),the ES-D3 transplanted group(group 4),the combination of ES-D3 and bFGF transplanted group(group 5).One week after the acute myocardial infarction(AMI),rats were injected with the in vitro differentiated ES-D3 labeled by BrdU.Then the cardiac function and histology were measured in week 4 after the transplantation. Results ES-D3 could differentiate into cardiomyocytes in vitro,and could survive steadily for 4 weeks in vivo after the transplantation into AMI’s model.There were no significant difference between group 2 and group 3 in cardiac function(P>0.05).Compared with group 2,the infarction size and left ventricular weight(LVW) were reduced significantly(P<0.01)in group 4 and group 5,and capillary density was increased significantly(P<0.01).The functions of the left ventricle were remarkably improved.Conclusion The transplantation of ES-D3 can promote angiogenesis,improve the cardiac function,prevent the remodeling process of the left ventricle and reduce the infarct size,and further benefits can be obtained by co-administration of bFGF.更多还原