【摘要】 目的:研究黄芩苷(BA)抑制Jurkat T细胞(人T淋巴细胞白血病细胞株)的生长和诱导其凋亡的作用及机制。方法:以噻唑兰(MTT)比色法测定BA对细胞生长的抑制率;胞内[Ca2+]i和线粒体跨膜电势(ΔΨm)分别用F luo-4/AM和D iOC6(3)荧光探针标记,流式细胞仪检测;PI染色流式细胞术分析细胞周期分布及凋亡率;凋亡细胞形态以Hoechst33342/PI染色后荧光显微镜观察。结果:BA明显抑制Jurkat细胞增殖(P<0.01),并呈时间、浓度依赖性;BA可浓度依赖的诱导Jurkat细胞[Ca2+]i浓度上升、线粒体膜电势ΔΨm降低,将细胞周期阻滞在S期,凋亡率增加,荧光显微镜下可见经BA(100 mg/L)作用的细胞呈现典型的凋亡核固缩表现,胞核呈致密浓染的颗粒状荧光。结论:BA体外显著抑制Jurkat T细胞增殖及诱导其凋亡,其机制可能与胞内[Ca2+]i及线粒体依赖的凋亡通路有关。更多还原

【Abstract】 Aim:To investigate the effects and mechanism of baicalin(BA) on growth inhibition and inducing apoptosis of Jurkat T cell(human T lymphocyte leukemia cell line) in vitro. Methods: The inhibitory effect of BA on growth of Jurkat cells was measured by MTT assay.Intracellular free Ca2+(i) and mitochondrial membrane potential(ΔΨm) in cells were labeled with Fluo-4/AM and 3,3’-dihexyloxycarbocyanine iodide [DiOC6(3)] and tested by flow cytometry(FCM).The distribution of the cell-cycle and apoptosis rates were analyzed by propidium iodide(PI) staining together with flow cytometry.Observe the morphological changes of the apoptosis cells by fluorescence microscope with Hoechst33342 and PI staining.Results: BA could inhibit the growth of Jurkat cells significantly in a time-and dose-dependent manner(P<0.01).BA induced apoptosis,i increase and the decrease of ΔΨm of Jurkat cells in a dose-dependent manner,and led the cell-cycle to S arrest(P<0.05).The morphological changes of apoptosis,such as nuclear fragmentation or condensed particles were identified after incubation by BA(100 mg/L).Conclusion:BA showed the effects on growth inhibition and inducing apoptosis of Jurkat cells,the mechanism of the action resulted in activating mitochondria and Ca2+ signaling pathway.更多还原