【摘要】 后基因组时代的到来使基因转录组学、蛋白质组学和代谢组学等高通量、多层次的生物研究手段广泛应用于植物科学研究,从海量数据中有效挖掘必要的生物信息成为当今组学研究的瓶颈问题。RiceCyc(http://www.gramene.org/pathway/)及其核心软件Pathway Tools是水稻代谢途径分析的有效工具。通过搜索TIGR(ht-tp://www.tigr.org/tdb/e2k1/osa1/)数据库,用Pathway Tools软件的omics viewer工具将耐盐品种FL478和其不耐盐亲本IR29在盐处理条件相对对照条件下的110个差异表达基因可视化展示在包含362条代谢途径信息的RiceCyc细胞代谢图上,从整体水平上分析了二者对盐胁迫反应的不同。结果表明,在相同的盐胁迫条件下,FL478相对IR29反应明显较快,前者中被诱导的代谢途径主要是能量循环过程如磷酸戊糖途径、糖酵解和三羧酸循环(TCA循环),以及核糖的分解、碳水化合物的利用途径;而后者中表达出现差异的基因主要涉及植物激素的生物合成,细胞结构组分代谢及次级代谢等途径;类黄酮合成途径在IR29中被明显诱导而在FL478中没有变化。更多还原

【Abstract】 Coming of post-genomic era announced the extensive application of high-throughput,non linear analysis like transcriptomics,proteomics and metabonomics in plant science,which led biological mining of mass experimental data to be one of the key problems in "omics" research.Systematic bioinformatics software Pathway tools and RiceCyc(http://www.gramene.org/pathway/) were excellent in rice metabolism pathway analysis.When searched against TIGR rice database(http://www.tigr.org/tdb/e2k1/osa1/),differently expressed genes in salt tolerant genotype FL478 and its salt-sensitive parent genotype IR29 under salinity stressed conditions comparing to control were visualized using omics viewer of Pathway tools and 110 of them were plotted in the cellular metabolism diagram involving 362 rice metabolism pathways.A bird’s eye view illustrated that genes involved in energy recycling processes like pentose phosphate pathway,fermentation and tricarboxylic acid cycle(TCA cycle),utilization pathways of ribose and carbohydrates were induced in salinity-stressed FL478,while in the samely treated IR29 genotype,genes related to biosynthesis pathways of plant hormones,cellular structural components and secondary metabolites were divergently expressed relative to control,which exhibited a swifter response to salinity stess of tolerant genotype FL478 than salt-sensitive genotype IR29.Significantly,flavonoid biosynthetic pathway was specifically induced in IR29 but not in FL478.更多还原