【摘要】 目的研究肝细胞癌(HCC)组织中C-JUN激活域连接蛋白1(JAB1)的表达及与P27kip1(P27)间的关系,探讨JAB1与临床病理及预后的关系。方法免疫组化检测HCC及相应癌旁肝组织中JAB1和P27的表达。Western blot及免疫沉淀检测JAB1和P27的表达及相互作用。结果HCC中,JAB1的表达高于癌旁肝组织,P27表达低于癌旁组织。JAB1的表达与组织分化程度、甲胎蛋白(AFP)值及转移相关(P<0.05)。HCC中JAB1与P27的表达呈负相关(r=-0.7077,P<0.001),并且能免疫共沉淀。结论JAB1的表达与P27负相关,JAB1作为P27的负性调控因子,可能与HCC的恶性进展及预后相关。更多还原

【Abstract】 Objective To investigate the expression of C-JUN activation domain binding protein 1(JAB1)and its relationship with expression of P27 protein in human hepatocellular carcinoma(HCC),and to determine whether JAB1 is associated with clinicopathological parameters and prognosis of HCC.Methods Immunohistochemical analysis was performed to investigate the expression of JAB1 and P27 in 76 cases of HCC and adjacent nontumorous tissues.Fresh tumor tissues and their adjacent nontumorous tissues from 8 cases of HCC were collected for Western blot and immunoprecipitation assays.Results The expression of JAB1 in HCC was significantly higher than that in adjacent nontumorous tissues.In contrast,P27 level was higher in nontumorous liver tissues than that in HCC.JAB1 overexpression was correlated with histological differentiation,serum alpha-fetoprotein(AFP)level and metastasis(P<0.05).In addition,a negative correlation between JAB1 and P27 expression was found in HCC(r=-0.7077,P<0.001),and co-immunoprecipitation experiments demonstrated a direct interaction between JAB1 and P27 in HCC.In both Kaplan-Meier Plot and multivariate Cox regression analysis,the overexpression of JAB1 was significantly associated with poor prognosis(P<0.05).Conclusion JAB1 expression is negcetively correlated with P27 expression,and JAB1,as a negative regulator of P27,may be associated with the progression and prognosis of HCC.更多还原