【摘要】 目的研究TSA对人肝癌SMMC-7721细胞增殖的影响。方法选用人肝癌SMMC-7721细胞作为实验对象,分为对照组和不同浓度TSA(0.1、0.5、1.0、2.0μM)处理组,倒置显微镜观察TSA对SMMC-7721细胞形态的影响,MTT比色法测定TSA对SMMC-7721细胞增殖的抑制情况,流式细胞术(FCM)分析细胞周期;结果倒置显微镜下,经TSA处理的细胞增殖速度显著减慢;MTT比色法测定结果显示不同浓度TSA对SMMC-7721细胞的增殖均有抑制作用,并有明显的剂量依赖和时间依赖关系;流式细胞仪检测结果显示,SMMC-7721细胞经TSA处理后,G0/G1期细胞明显增加,S期细胞则明显减少,细胞被阻滞于G0/G1期。结论TSA通过抑制HDAC的活性,调节相关基因的转录,从而调控细胞周期,有效抑制肝癌细胞的增殖。更多还原

【Abstract】 Objective To investigate the effects of TSA on proliferation of the human hepatocarcinoma SMMC-7721 cells.Methods After the human hepatocarcinoma cells had been treated with various concentrations of TSA(0.1、0.5、1.0、2.0 μM)in different duration in vitro,cell morphology was observed by the inverted light microscope,and cell viability was analyzed by MTT assay,and the cell cycle was investigated by flow cytometry(FCM).Results TSA inhibited the proliferation of SMMC-7721 cells in dose-dependent and time-dependent manners.FCM analysis showed TSA could arrest SMMC-7721 cells in G0/G1 phase,with accumulation of the cells in G0/G1 phase and with decrease of the cells in S phase.Conclusion TSA could inhibit the proliferation of the hepatocarcinoma SMMC-7721 cells,which were associated with the inhibition of enzymatic activity of HDAC,regulation of relative gene transcriptions and the G0/G1 arrest.更多还原