【摘要】 目的建立高效液相色谱法测定小儿解表颗粒中绿原酸含量的方法。方法采用Hypersil C18(250mm×4.6mm,5μm),色谱柱;以乙腈-0.4%磷酸溶液(13∶87)为流动相,流速为1.0mL/min,柱温为室温,检测波长为327nm,以外标法测定金银花药材及小儿解表颗粒中绿原酸的含量。结果绿原酸在11.2~134.4μg.mL-1内呈线性关系。绿原酸在制剂中的平均回收率(n=5)为99.18%(RSD=0.60%)。结论该方法准确、简便、重现性好,可用于小儿解表颗粒的质量控制。更多还原

【Abstract】 Objective:To establish the method of determination of chlorogenic acid in Flos Lonicerae and Xiaoer Jiebiao granules by HPLC.Methods:An Hypersil C18 was used with a mobile phase consisted of mixture of acetonitrile-0.4% phosphoric acid(13∶87) at the flow rate of 1.0mL/min in this method.And UV detector wavelength was 327nm.Results:The linear range of chlorogenic acid was 11.2~134.4μg· mL-1(r=0.9999).The average recovery was 99.18%(RSD=0.60%,n=5).Conclusion:An accurate,simple,rapid qualitative method with good reproducibility to assay chlorogenic acid in Flos Lonicerae raw material and Xiaoer Jiebiao granules is established.更多还原