【摘要】 利用RT-PCR方法从铜绿微囊藻PCC7806 mcy基因中扩增出目的基因片段,用TA克隆方法将基因片段插入pGEMT-T Easy中来制备质粒标准品.通过不同连续稀释倍数的质粒标准品成功构建了16srRNA和mcyH两基因的标准曲线,该法构建的两基因标准曲线r2分别为0.9916和0.9938,且质粒标准品具有较好的重复性,满足实时荧光定量RT-PCR的要求.更多还原

【Abstract】 Microcystis aeruginosa 7806 mcy genes RT-PCR product was subcloned into PGEM-T vector to contrust recombinant plasmid.Standard curves of 16srRNA and mcyH were developped by different folds serial diluted dplasmid DNA,and r2of two standard curves separately was 0.9916 and 0.9938 by the method,The plasmid standard has a better reproducibility.It can satisfy the experiments need.更多还原