【摘要】 借鉴互联网中已克隆的活化素βA亚基基因的序列,设计并合成一对兼并引物,首次扩增和克隆到亚洲黑熊βA亚基基因的357 bp片段,以水作为阴性对照排除污染,多次重复实验获得一致稳定结果.序列分析显示此片段在处于不同进化程度的物种之间,仍然具有高度的保守性,亚洲黑熊βA亚基基因与人相比有29个核苷酸差异,一致性高达91.6%;与亲缘关系较近的大熊猫、小熊猫和马来熊相比,分别有4、20、3个核苷酸差异,一致性分别为98.9%、94.4%、99.2%.系统发育分析和酶切图谱分析显示亚洲黑熊、大熊猫和马来熊具有较近的亲缘关系,而它们与小熊猫的亲缘关系较远.更多还原

【Abstract】 The 357-bp DNA coding sequence of the Activin βA subunit gene was cloned for the first time from Asiatic Black Bear(Selenarctos thibetanus)with degenerate primers based on the reported sequence of other species Activin βA subunit gene.Water was used as a negative control and the experiments were repeated several times to obtain reliable and identical results.Sequence analysis showed that the Activin βA subunit gene of Selenarctos thibetanus was highly conserved compared to other species that have different evolution levels,with 29 bp changes to human.There have 4,20,3 base pairs changes separately compared to giant panda(Ailuropoda melanoleuca),lesser panda(Ailurus fulgens) and sun bear(Helarctos malayanus),with identities of 98.9%,94.4%,99.2% respectively.Analysis of phylogenisis and cleavage site,the Selenarctos thibetanus,giant panda and sun bear appeared to be phylogenetically closer but all have a remote relationship with the lesser panda.更多还原