【摘要】 目的研究二烯丙基二硫(DADS)诱导白血病细胞凋亡及其机制。方法实验分对照组、DADS处理组、Ac-DEVD-CHO预处理组,进行体外细胞培养,采用形态学观察、MTT法、流式细胞术、DNA凝胶电泳等方法,进行细胞形态学观察、细胞增殖抑制作用研究,检测细胞凋亡率、Caspase-3活性以及DNA Ladder检测。结果DADS诱导前后,HL-60细胞形态学发生明显改变,DADS以剂量依赖的方式抑制细胞的生长,在DADS诱导HL-60细胞凋亡过程中有Caspase-3的活化,且与剂量和作用时间呈依赖关系,Caspase-3抑制剂Ac-DEVD-CHO能抑制DADS诱导HL-60细胞凋亡。结论DADS能有效地诱导HL-60细胞凋亡,其过程与Caspase-3活化相关。更多还原

【Abstract】 Objective To investigate the apoptosis in human leukemia HL-60 cells induced by DADS,and study the mechanism. Methods HL-60 cells were treated with different concentrations of DADS.After incubation of HL-60 cells with DADS,the effects of DADS on HL-60 cell growth inhibition were estimated by morphology and MTT assay,flow cytometry was used to determine the induction of apoptosis and the expression of actived Caspase-3,DNA agarose electrophoresis was used to determine the induction of apoptosis.Results After HL60 cells were exposed to DADS,the typical apoptotic morphological changes were observed under the light microscope and inverted microscope,DADS inhibited the growth of HL-60 cells in a dose-dependent manner,when HL-60 cells induced by DADS the expression of actived Caspase-3 was increased in a dose-and time-dependent manner.Pretreatment with Caspase-3 specific inhibitor Ac-DEVD-CHO prevented DADS induced apoptosis. Conclusion DADS can efficiently induce apoptosis in HL60 cells and the activation of Caspase-3 may contribute to the effect.更多还原