【摘要】 目的探求不同幅值脉冲电刺激对内皮细胞的形态、增殖以及活性物质NO和ET-1分泌量的影响。方法选择在50Hz频率,幅值分别为25mV,50mV,100mV,200mV,400mV,800mV,1600mV,3200mV的电刺激6h作用于内皮细胞。用扫描电镜观察细胞形态。检测MTT,分析细胞增殖。检测内皮细胞NO和ET-1的分泌量。结果在低幅值脉冲电刺激(25mV,50Hz)作用下,内皮细胞明显增殖,同时大量伪足突起出现,分泌物也同时增多。但当电刺激高于100mV后,脉冲电刺激明显抑制细胞生长。NO的表达在电刺激作用后明显增加,随电刺激幅值增加而提高,在400mV左右出现峰值。ET-1的表达在25mV出现峰值后逐渐下降。结论脉冲电刺激对内皮细胞的形态、增殖、ET-1和NO表达都有影响。同时,预示低幅值脉冲电刺激是有效辅助血管生成的手段。更多还原

【Abstract】 Objective To find out the effect of different amplitudes pulsed electrical stimulation(PES) on form,proliferation and secreting amounts of NO and endothelin-1(ET-1) of endothelial cells(ECs).Methods Under 50 Hz,various different amplitudes PESs(25 mV,50 mV,100 mV,200 mV,400 mV,800 mV,1 600 mV,3 200 mV) acted on ECs for 6 h respectively.The cell forms were observed with scanning electron microscope(SEM).The proliferation was analyzed with OD value of MTT method.NO and ET-1 amounts of ECs were detected.Results The proliferation of the ECs was obviously rose up under the low amplitude PES(25 mV,50 Hz).At the same time,many pseudopodia appeared.But the PES inhibited obviously the proliferation with the amplitude higher than 100 mV.After the PES,the expression of NO increased more obviously following the amplitude increased.Its peak appeared at 400 mV.The peak of ET-1 appeared at 25 mV,then gradually decreased.Conclusion The PES has significant effect on the ECs’ form,proliferation and the expressions of NO and ET-1.It is indicated that the low amplitude PES is a useful method to promote angiogenesis.更多还原