【摘要】 对厦门和湛江海域的条纹斑竹鲨[Chiloscyllium plagiosum(Bennett,1830)]的线粒体DNA控制区和细胞色素b(Cytb)基因进行PCR扩增和测序,获得线粒体DNA控制区、Cytb基因和tRNAPro基因全序列(GenBank序列号:EU363740~EU363752)。结果表明,tRNAPro基因长度为69bp,控制区长度为1094~1096bp,Cytb基因全序列长度为1146bp。tRNAPro基因非常保守,未发现碱基变异。所测30个个体中,控制区只有4个多态位点,5种不同的单倍型;Cytb基因全序列共有10个多态性位点,7种单倍型。Cytb基因的1146个碱基编码381个氨基酸,氨基酸链共有3个变异位点,4种单倍型,厦门群体具有4种单倍型,湛江群体只有1种单倍型。综合Cytb基因和控制区序列,2个条纹斑竹鲨群体共有10种单倍型,其中厦门群体有9种单倍型,湛江群体仅4种单倍型。研究结果表明条纹斑竹鲨在控制区和Cytb基因序列上的遗传多样性均较低,厦门群体的遗传多样性水平高于湛江群体。更多还原

【Abstract】 Genetic diversities of Chiloscyllium plagiosum(Bennett,1830)from Xiamen and Zhanjiang were investigated with mitochondrial control region and cytochrome b(Cyt b)gene sequence analysis.Control region and Cyt b gene fragments from 30 individuals were cloned with PCR method and sequenced.The sequences of the mitochondrial control region,Cyt b gene and tRNAPro gene were obtained.The complete sequences of the mitochondrial control region,Cyt b gene and tRNAPro gene were 69 bp,1 094～1 096 bp and 1 146 bp respectively.tRNAPro gene seemed conservative,without any polymorphism.Among 30 samples,there were 4 polymorphyism sites with 5 haplotypes for control region sequence and 10 polymorphyism sites with 7 haplotypes for Cyt b gene sequence.1 146 bp nucleotide of Cyt b gene encoded 381 amino acids,and the amino acid chain had 3 polymorphyism sites with 4 haplotypes.Samples of Xiamen stock had 4 haplotypes while Zhanjiang stock only 1 haplotype.It was seemed that the genetic diversities in Cyt b and control region from C.plagiosum in Xiamen were comparatively higher than those in Zhanjiang,though both appeared relatively low.更多还原