【摘要】 为研制和生产鲈鱼苗疫苗提供依据,采用人工感染试验方法测试了花鲈(Lateolabrax japonicus)烂尾、出血病病原菌W-1菌株(鳗弧菌Vibrio anguillarum)对花鲈苗的半致死浓度(LD50)及不同保存条件下的W-1菌株的致病力。试验结果表明:W-1菌浴感染花鲈苗的半致死浓度为2.5×107.5个/mL,针刺后菌浴感染花鲈苗的半致死浓度为2.5×107个/mL;该病原菌为一条件致病菌,创伤感染是主要的致病途径;常温多次传代、15℃、-20℃和-80℃等不同保存条件下W-1菌株的致病力均很强,但常温多次传代保存的菌株致病力比其他3种方法保存的菌株致病力稍差一些。更多还原

【Abstract】 The lethal dose for 50%(LD50) of Vibrio anguillarum W-1 in seaperch fry and the pathogenicity of strain W-1 stored in different conditions were investigated in this paper.The results showed that the LD50 for seaperch fry with immersion infection was 2.5×107.5cell/mL while the LD50 for the seaperch fry challenged with immersion after abdominal puncture was 2.5×107cell/mL.The pathogen W-1 was an opportunistic pathogen and injury was the main infection factor.In addition,the strain W-1 reproduced many times as well as the strain W-1 stored at 15℃,-20℃ and-80℃ had a strong pathogenicity to the seaperch fry.更多还原