【摘要】 为调查整合子在胶州湾水域多重耐药菌中的分布情况。本文采用PCR、克隆测序等方法对多重耐药菌株中I、II型整合酶及整合子中耐药基因盒的分布进行检测。研究发现,胶州湾多抗性细菌污染严重50%的多重耐药菌携带整合子;整合子阳性菌株对多种抗生素的耐药率远大于整合子阴性菌;二氢叶酸还原酶和氨基糖苷腺苷转移酶是基因盒携带的主要耐药基因;基因盒-整合子系统参与了细菌多重耐药的形成,已成为水环境中多重耐药基因水平传播的重要机制。更多还原

【Abstract】 The investigate of distribution of integron in multidrug resistance isolates in Jiaozhou Bay was carried out.The presence of Class 1 and 2 integrase genes were determined by PCR with integrase specific primers.Clone and DNA sequencing were used to analyze the resistance-related gene cassettes.50% of multidrug resistance isolates were detected with integron.The multidrug resistance rate of positive-integron isolates is far greater than the negative ones.The dfr and aadA were predominant genes in integron.The integron-mediated horizontal transference plays a particularly important role in the spread of multidrug resistance in aquatic environments.更多还原