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Effect of lead on cytoskeletal protein stability in crucian carp Carassius auratus

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【作者】 成嘉张东裔褚武英刘芳刘臻周瑞雪蒙涛张建社

【Author】 CHENG Jia~+ ZHANG Dongyi CHU Wuying LIU Fang~+ LIU Zhen ZHOU Ruixue MENG Tao ZHANG Jianshe~(+**) + Department of Bioengineering and Environmental Science,Changsha University,Changsha 410003,China ++ Core Facilities of Biotechnology,Central South University of Forestry and Technology,Changsha 410004,China

【机构】 Department of Bioengineering and Environmental Science,Changsha UniversityCore Facilities of Biotechnology,Central South University of Forestry and Technology

【摘要】 <正>Inorganic lead (Pb) is one of the most common environmental pollutants.Much evidence indicates that Pb exposure could directly affect fish growth and development.In this study,we investigated the cytotoxic effects of Pb on cytoskeletal protein stability at both protein and mRNA level in crucian carp Carassius auratus.Pb(NO32 treatment in concentration of 100μmol/L resulted in decreased expression of bothα-andβ-tubulin but y-tubulin as assayed with SDS-PAGE,Western Blot, and ELISA.In vivo and in vitro analyses on protein expression of tubulins are consistent.The effect of Pb on mRNA expression varied among different tissues.Our results suggest that cytotoxicity of Pb at protein translation level is stronger than at mRNA expression level.

【Abstract】 Inorganic lead (Pb) is one of the most common environmental pollutants.Much evidence indicates that Pb exposure could directly affect fish growth and development.In this study,we investigated the cytotoxic effects of Pb on cytoskeletal protein stability at both protein and mRNA level in crucian carp Carassius auratus.Pb(NO32 treatment in concentration of 100μmol/L resulted in decreased expression of bothα-andβ-tubulin but y-tubulin as assayed with SDS-PAGE,Western Blot, and ELISA.In vivo and in vitro analyses on protein expression of tubulins are consistent.The effect of Pb on mRNA expression varied among different tissues.Our results suggest that cytotoxicity of Pb at protein translation level is stronger than at mRNA expression level.

【基金】 Supported by National Natural Science Foundation of China (No.30571414,No 30640015);Changsha Municipal Project of Science and Technology (No.K051127-72).
  • 【文献出处】 Chinese Journal of Oceanology and Limnology ,中国海洋湖沼学报(英文版) , 编辑部邮箱 ,2008年04期
  • 【分类号】X174
  • 【下载频次】44
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