【摘要】 目的研究c-abl基因在人卵巢肿瘤组织中的突变情况及其临床意义,探讨其在卵巢肿瘤的发生、发展过程中的作用。方法应用PCR技术及序列分析方法检测和分析88例正常卵巢、上皮性肿瘤组织中c-abl基因在外显子3、4区的突变。结果①c-abl基因在外显子3、4区的突变总体上表现为点突变。卵巢恶性肿瘤组织及交界性肿瘤的突变率较正常及良性肿瘤组高;②卵巢恶性上皮性肿瘤组织中,突变率与病理类型无关,随肿瘤组织分化、临床分期、淋巴结转移的进展而增高;③在卵巢恶性上皮性肿瘤组织中,c-abl基因在外显子3、4的点突变集中在5个位点,它们或单独或共同存在,影响该蛋白的结构和功能。结论c-abl基因在外显子3、4区的突变与卵巢上皮性癌的分化、临床分期和淋巴结的转移呈正相关,可成为判断该肿瘤恶性度的指标之一。更多还原

【Abstract】 Objective To investigate the mutations of gene c-abl and their significance in the ovarian tumors and analyze the relationship of its mutations with the development of the ovarian tumors.Methods The mutations of gene c-abl in exon 3 and 4 were studied with PCR and sequencing technique in 5 normal ovarian tissues,20 benign ovarian epithelial tumors,16 ovarian borderline epithelial tumors and 47 ovarian epithelial carcinomas.Results ①The mutations of gene c-abl in exon 3 and 4 in 88 normal ovarian tissues and ovarian epithelial tumors presented point mutation.The mutation rate of the borderline epithelial tumors was statistically higher than that of normal and benign group(P<0.05),and that of carcinoma was the highest(P<0.05);②In 47 ovarian epithelial carcinomas,there was no difference in the mutation rates during the different pathological types;and there were distinct differences during the groups of different differentiated grade,the development of FIGO clinical stages and lymphatic metastasis;③There were 5 mutation sites existing solely or together in exon 3 and 4.Conclusion The mutation of c-abl in exon 3 and 4 relates to the differentiation,the clinical FIGO staging and lymphatic metastasis,and the gene could be the one of indexes that indicates the malignant grade of the ovarian tumors in clinics.更多还原