【摘要】 目的:探讨基质金属蛋白酶2(MMP-2)mRNA在人喉鳞状细胞癌中的定位及临床意义。方法:对50例喉癌患者的原发灶50例,癌旁组织34例,正常组织22例,采用原位杂交方法检测MMP-2 mRNA在喉癌组织中的定位及表达情况,通过图像分析研究其表达量与临床病理参数(T分期、病理分级、淋巴结转移)的关系。结果:MMP-2 mRNA在肿瘤细胞和基质细胞的胞浆中表达,其表达量在原发灶、癌旁(淋巴结)、正常组织中的表达呈递减趋势;与肿瘤的病理分级、T分期、淋巴结的转移无关。结论:MMP-2 mRNA在喉癌组织的肿瘤细胞和基质细胞的胞浆中表达,在原发灶中表达最高,其量的表达与病理分级、T分期、淋巴结的转移无关。可能是肿瘤浸润转移的一个早期信号。更多还原

【Abstract】 Objective:To study the localization and expression of matrix metalloproteinase-2(MMP-2)mRNA in human laryngeal squamous cell carcinoma tissue.Methods:The amount of expression of 50 laryngeal carcinoma patients,50 cases primary tissues lateral carcinoma,lymph node,20 cases normal tissue was analyzed by the immunohistochemical analysis.The MMP-2 protein in ISH(immunohistochemistry)was evaluated by the image analysis.In addition,the correlation between the MMP-2 mRNA expression and clinicopathological features(pathology grades,T stages,lymph node metastasis)wasevaluated.Results:The MMP-2mRNA was observed in carcinoma cell and stromal cytoplasm,Although the MMP-2mRNA expression in laryngeal carcinoma tissues was high than the lateral tissues,it was not correlated with clinicopathological features(the tumor T stage,pathology grades,lymph node metastasis).Conclusion:The MMP-2mRNA was observed in carcinoma cell and stromal cytoplasm.MMP-2mRNA expression in the primary tissues in laryngeal carcinoma was the highest among these samples,it maybe an early information of carcinoma invasion and lymph node metastasis.更多还原