【摘要】 目的采用HPLC法测定甲睾酮喷雾剂的含量。方法色谱柱为Shimpack VP C18柱(150 mm×4.6 mm,5μm);流动相为甲醇-水(65:35);检测波长241 nm。结果甲睾酮0.052~4.160μg与峰面积的线性良好(r=0.9999);平均回收率为99.86%(RSD=0.98%,n=9)。结论所建方法简便、灵敏、准确,可用于甲睾酮喷雾剂的含量测定。更多还原

【Abstract】 OBJECTIVE To establish a method to determine methyltestosterone in Methyltestosterone spray by HPLC.METHODSShimpack VP C18(150 mm×4.6 mm,5 μm) column was used with the mobile phase consisted of methanol-water(65:35).The ultraviolet detector was set at 241 nm.RESULTS A good linearity was obtained in the range of 0.052-4.160 μg(r=0.9999).The average recovery was 99.86%(n=9) with RSD of 0.98%.CONCLUSIONThe method is simple,sensitive and applicable for the determination of methyltestosterone in Methyltestosterone spray.更多还原