【摘要】 目的研究叶枯唑原药对亚慢性染毒大鼠甲状腺的影响。方法100只SD大鼠随机分为对照组和4个不同剂量叶枯唑组,分别给予0、7.0、27.9、111.7、447.0 mg/kg叶枯唑原药灌胃90 d。实验结束取甲状腺称重并测定脏器系数,光镜下观察甲状腺组织学改变,免疫组化测定增殖细胞核抗原(PCNA)在甲状腺中的表达。结果27.9、111.7和447.0 mg/kg叶枯唑组甲状腺湿重和脏器系数高于对照组(P<0.01);组织学观察见甲状腺滤泡增生,但增生表现不一致;27.9、111.7和447.0 mg/kg叶枯唑组PCNA阳性细胞数多于对照组(P<0.05或P<0.01)。结论长期染毒叶枯唑原药能引起大鼠甲状腺增生,其机制有待进一步研究。更多还原

【Abstract】 Objective To test the impact of subchronically administered Bismerthlazol on the thyroid morphosis of rats.Methods One hundred SD rats were randomly divided into one negative control group and four experimental groups with 7.0,27.9,111.7,and 447.0 mg/kg daily doses of Bismerthlazol,respectively.The Bismerthlazol was administered by gavage for 90 d.At the end of the experiment,the thyroids of the rats were harvested and weighted.The pathological changes of the thyroids were observed under light microscopes.The positive expression of PCNA in the thyroid glands were examined by histochemistry methods.Results Increased coefficients of thyroid gland weight were found in the experimental groups(P<0.01).The thyroid glands showed different hyperplasia of follicular cells.Increased positive cells of PCNA were observed in the experimental groups(P<0.05 or P<0.01) except for the 7.0 mg/kg dose group.Conclusion Long-time administered Bismerthlazol causes thyroids hyperplasia in rats.Further study on the mechanisms is warranted.更多还原