【摘要】 目的观察2日龄(P2)新生大鼠脑白质损害(WMD)后神经胶质细胞凋亡及丝裂原活化蛋白激酶活化蛋白激酶2(MK2)、Nogo-B的变化;探讨神经胶质细胞凋亡与MK2、Nogo-B变化的时相关系。方法制备新生鼠WMD模型,分别于WMD后30 min、1 h、4 h、12 h、1 d、3 d、7 d、14 d及21 d处死大鼠,TUNEL法检测脑白质神经细胞凋亡,免疫组化(SP)法检测Nogo-B蛋白的表达,原位杂交(POD法)检测MK2 mRNA表达。结果WMD组大鼠凋亡指数在缺氧缺血4 h、12 h、1 d、3 d、7 d组与对照组相比,差异有统计学意义(P<0.05);WMD后1 h MK2mRNA在脑室周围白质及胼胝体区表达上调,并于损伤后3 d达到高峰。Nogo-B在WMD后12 h表达增加,3 d达高峰,在12 h、1 d、3 d、7 d的表达与对照组相比,差异有统计学意义(P<0.05)。结论MK2、Nogo-B在新生大鼠脑白质损害时表达增高,提示其参与了脑白质损害。更多还原

【Abstract】 Objective To observe and explore the apoptosis of neuroglial cell and the expression changes of neurite outgrowth inhibitor B(Nogo-B) and MAP kinase-activated protein kinase 2(MK2) in SD rats with white matter damage(WMD).Methods The WMD model of newborn rat was established for this study,and then the pups were respectively killed at 30 min,1 h,4 h,12 h,1 d,3 d,7 d,14 d,21 d after WMD.The apoptosis of neuroglial cells was investigated by TUNEL method;the changes of Nogo-B expression in white matter were investigated by immunohistochemical technique;the changes of expression of MK2 mRNA in white matter were measured by in situ hybridization.Results TUNEL result showed the number of apoptotic cells increased at 4 h and peaked at 3 d,decreased at 7 d after WMD of newborn rat;the expression of MK2 mRNA began to increase at 1 h and reached a maximum at 3 d(P<0.05);the expression of Nogo-B in periventricular white matter and corpus callosum of WMD rat was up-regulated at 12 h and peaked at 3 d after cerebral white matter damage,displaying significant differences at 12 h,1 d,3 d and 7 d when the Nogo-B expression in WMD rat compared with that observed in the control(P<0.05).Conclusion Nogo-B and MK2 can participate in pathogenesis of white matter damage in newborn rat.更多还原