【摘要】 目的:比较不同产地、不同品种的枳实中总生物碱与挥发油含量差异。方法:枳实中粉用甲醇提取,提取液蒸干,加水溶解,过聚酰胺柱,用水洗脱,定容至25 mL,取出5.0 mL,加0.2 mol/L NaOH5.0 mL,定容至10 mL,于292 nm处测定样品吸光度A值,按回归方程计算总生物碱的百分含量;以《中国药典》法测定挥发油含量。结果:湘枳实中总生物碱和挥发油含量最高,分别为0.86%与1.02%;赣枳实(0.82%、0.96%)与绿衣枳实(0.84%、0.98%)稍低一点,但3者无明显差异;苏枳实次之(0.75%、0.83%),甜橙枳实最低(0.41%、0.57%)。结论:该方法简便、快速、稳定、重复性好、回收率高,枳实作药用以湘枳实、赣枳实和绿衣枳实为佳。更多还原

【Abstract】 Objective: To compare the difference of total alkaloids and aetherolea in different species and producing area of aurantii fructus immaturus.Methods: the power of aurantii fructus immaturus were extracted by methanol,evaporating extract to dryness,dissolving with adding water,passing polyamide column,eluting by water,metered volume to 25 mL,taking out of 5 mL from it,adding 5.0 mL NaOH(0.2 mol/L),metered volume to 10 mL,after that,the absorbance A of samples were determined at 292 nm,the total alkaloids were calculated by regression equation,the contents of aetherolea were determined with the method of Chinese Pharmacopeia.Results: the total alkaloids and aetherolea were the highest in aurantii fructus immaturus of Hunan province,achieving to 0.86% and 1.02% respectively,the contents were lower in that of Jiangxi province(0.82%,0.96%) and green aurantii fructus immaturus(0.84%,0.98%),but the difference has no statistical significance among three species.The contents of that in Su aurantii fructus immaturus was 0.75% to 0.83%,The contents of that were the lowest in cirtus sinensis aurantii fructus immaturus(0.41%,0.57%).Conclusion: The method is simple,reliable and reproducible,the aurantii fructus immaturus of Hunan and Jiangxi province,green aurantii fructus immaturus have better effect as the drugs.更多还原