【摘要】 目的:建立熟大黄中没食子酸含量测定方法。方法:采用高效液相色谱法,色谱柱:Diamonsil C18(250 mm×4.6 mm,5μm);流动相:甲醇-0.01%磷酸(12∶88);流速:1 ml.min-1;柱温:30℃;检测波长:273 nm。结果:没食子酸在0.0224～0.448μg范围内线性关系良好,平均回收率为99.26%,RSD%为2.17%。结论:该方法简便可行、重复性好,可作为大黄炮制品质量评价的依据。更多还原

【Abstract】 Objective: To establish a HPLC method for the determination of garlic acid in Radix et Rhizoma Rhei.Methods: A HPLC-UV method with the detected wave length of 273 nm and a Diamonsil C18(250 mm×4.6 mm,5μm) column was used,the mobile phase was MeOH-0.01% H3PO4(10∶90) with the flow rate was 1 mL·min-1.Results: A good linearity of garlic acid was in the range of 0.0224~0.448 μg,the average recovery was 99.26%.Conclusion: The method is rapid,sensitive and accurate.It can be used to control the quality of Radix et Rhizoma Rhei in different processed products.更多还原