【摘要】 【目的】探讨转化生长因子β1(TGF-β1)反义寡核苷酸(antisense oligonucleotides,ASODN)在肾纤维化发病机制中的作用。【方法】采用RT-PCR、Western blot检测脂质体介导的TGF-β1ASODN目的基因及蛋白的表达;通过细胞生长试验、流式细胞仪检测TGF-β1ASODN转染前后细胞增殖和凋亡的变化。【结果】转染后可见TGF-β1的基因及蛋白表达明显下调;和对照组相比,可明显抑制成纤维细胞的增殖,诱导其凋亡(P<0.01)。【结论】转化生长因子β1反义寡核苷酸对肾间质成纤维细胞具有明显的抑制作用,转染脂质体介导的TGF-β1ASODN在治疗肾纤维化探讨中可能具有重要意义。更多还原

【Abstract】 【Objective】To investigate the effect of transforming growth factor-β1(TGF-β1)on the proliferation and apoptosis of renal interstitial cell.【Methods】 HEK293 cell line was treated with TGF-β1 antisense oligodeoxynucleotides(ASODN) by lipofectamine.The inhibition of proliferation and apoptosis of the cell were studied with cell growth curves,MTT method and flow cytometry.The expression of TGF-β1 was detected by RT-PCR and Western blot respectively.【Results】After transfection,the mRNA and protein expression of TGF-β1 in the cells were significantly decreased or negative expression.Compared with the control group,TGF-β1 significantly inhibited cell proliferation and induced cell apoptosis(P<0.01).【Conclusion】 TGF-β1 ASODN may significantly inhibit the proliferation and induce apoptosis of renal interstitial cell.These results suggest that infected TGF-β1 ASODN by lipofectamine has an important role in the treatment of renal fibrosis.更多还原