【摘要】 目的:观察氯化锰(MnCl2)对去势大鼠生殖内分泌功能的影响,探讨MnCl2的抗雄激素样作用。方法:采用Hershberger体内筛检实验。对大鼠行睾丸摘除术去势,7 d后随机分为6组进行实验,溶剂对照组皮下注射玉米油,余5组大鼠皮下注射丙酸睾酮(TP)1.0μg/只,阴性对照组、低、中、高剂量染毒组同时腹腔注射MnCl20、7.5、15.0、30.0 mg/kg,阳性对照组经口灌胃氟他胺,每d 1次,连续7 d。7 d后,用放射免疫方法测定血清睾酮(T)浓度,免疫放射法测定前列腺特异抗原(PSA)含量;分离雄激素依赖器官称量,计算脏器系数。结果:去势后7 d,各组大鼠恢复良好,未观察到明显中毒症状。各实验组大鼠体质量变化与溶剂对照组比较差异无统计学意义(P>0.05)。氯化锰30 mg/kg剂量组的腹侧前列腺和精囊腺脏器系数低于阴性对照组(P<0.05)。染毒组去势大鼠血清T、PSA水平高于阳性对照组(P<0.05),与阴性对照组比较,差异无统计学意义(P>0.05)。结论:氯化锰在30 mg/kg此剂量下可能具有抗雄激素作用。更多还原

【Abstract】 Aim: To study the antiandrogenic effect of MnCl2 examined with Hershberger test.Methods:By the Hershberger assay,adult health SD male rats were castrated,and 7 days later,1.0 μg/rat testosterone propionate(TP) was administered by subcutaneous injection.MnCl2 was administered at doses of 0 mg/kg(the negtive control group),7.5 mg/kg,15 mg/kg,and 30 mg/kg by intraperitoneal injection.And flutamide(Flu) was given orally at a dose of 100 mg/kg to the positive control group.All rats were exposed to these exogenous substances for 7 days.Then the rats were sacrificed 24 hours after the last administration.The tissues depended on androgen were weighed using analytic balance,and testosterone(T) and prostate specific antigen(PSA) in serume were analysed using radioimmunity and immradiation.Results:The weight of rats didn’t differ from each other(P>0.05).The serum levels of T and PSA of the rats administered MnCl2 were higher than those of the positive conrol group(P<0.05),but didn’t differ from those of the negative control group(P>0.05).The organ coefficient of ventro-prostate and seminal vesicle of the rats administered MnCl2 30 mg/kg were lower than those of the negative control group(P<0.05).Conclusion: MnCl2 may have the antiandrogenic properties on rat in vivo screening tests.更多还原